Engineered zinc finger proteins for manipulation of the human mitochondrial genome.

TitleEngineered zinc finger proteins for manipulation of the human mitochondrial genome.
Publication TypeJournal Article
Year of Publication2010
AuthorsMinczuk, M
JournalMethods Mol Biol
Volume649
Pagination257-70
Date Published2010
ISSN1940-6029
KeywordsCell Line, Tumor, Endonucleases, Fluorescent Antibody Technique, Genetic Therapy, Genome, Mitochondrial, Humans, Mitochondrial Diseases, Protein Engineering, Zinc Fingers
Abstract

Currently there are no effective methods to manipulate or modify particular sequences in mammalian mitochondrial DNA (mtDNA) within cells. The availability of such methods would be of great value for basic mitochondrial research and would aid in development of therapies for diseases linked with mutations in mtDNA. Engineered zinc finger proteins have been used as powerful tools for intervening in nuclear gene expression and modifying nuclear DNA in a sequence-specific manner. Here, basic methods that are helpful in adapting the engineered zinc finger technology for targeting the DNA in mitochondria are presented with the main emphasis on mitochondrial import of zinc finger proteins.

DOI10.1007/978-1-60761-753-2_16
Alternate JournalMethods Mol. Biol.
Citation Key10.1007/978-1-60761-753-2_16
PubMed ID20680840
Grant ListMC_U105697135 / / Medical Research Council / United Kingdom