|Title||Overexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase.|
|Publication Type||Journal Article|
|Year of Publication||2002|
|Authors||Minczuk, M, Dmochowska, A, Palczewska, M, Stepien, PP|
|Date Published||2002 Nov|
|Keywords||DEAD-box RNA Helicases, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Fungal, Mitochondria, RNA, RNA Helicases, RNA, Fungal, RNA, Mitochondrial, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins|
RNA helicase, encoded by the Saccharomyces cerevisiae nuclear gene SUV3, is a subunit of the mitochondrial (mt) degradosome: an enzyme complex that takes part in turnover of mtRNAs. Deletion of the SUV3 gene leads to a variety of disturbances in mtRNA metabolism and results in respiratory incompetence of yeast cells. Here we show that the nuclear gene MSS116, which codes for a mitochondrial putative RNA helicase necessary for splicing of several mt introns, can suppress the lack of the SUV3 gene. Overexpression of the Mss116 putative helicase from a multicopy plasmid present in the SUV3-deleted strains partially restores respiratory competence, brings the steady-state levels of COB and ATP6/8 mRNA back almost to normal and lowers the accumulation of 21S rRNA and ATP6/8 RNA precursors to the wild-type levels. To the best of our knowledge, this is the first reported case of a substitution of one RNA helicase by another, belonging to a different class of RNA helicases.