Overexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase.

TitleOverexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase.
Publication TypeJournal Article
Year of Publication2002
AuthorsMinczuk, M, Dmochowska, A, Palczewska, M, Stepien, PP
JournalYeast
Volume19
Issue15
Pagination1285-93
Date Published2002 Nov
ISSN0749-503X
KeywordsDEAD-box RNA Helicases, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Fungal, Mitochondria, RNA, RNA Helicases, RNA, Fungal, RNA, Mitochondrial, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Abstract

RNA helicase, encoded by the Saccharomyces cerevisiae nuclear gene SUV3, is a subunit of the mitochondrial (mt) degradosome: an enzyme complex that takes part in turnover of mtRNAs. Deletion of the SUV3 gene leads to a variety of disturbances in mtRNA metabolism and results in respiratory incompetence of yeast cells. Here we show that the nuclear gene MSS116, which codes for a mitochondrial putative RNA helicase necessary for splicing of several mt introns, can suppress the lack of the SUV3 gene. Overexpression of the Mss116 putative helicase from a multicopy plasmid present in the SUV3-deleted strains partially restores respiratory competence, brings the steady-state levels of COB and ATP6/8 mRNA back almost to normal and lowers the accumulation of 21S rRNA and ATP6/8 RNA precursors to the wild-type levels. To the best of our knowledge, this is the first reported case of a substitution of one RNA helicase by another, belonging to a different class of RNA helicases.

DOI10.1002/yea.906
Alternate JournalYeast
Citation Key10.1002/yea.906
PubMed ID12402239