Overcoming barriers to membrane protein structure determination.

TitleOvercoming barriers to membrane protein structure determination.
Publication TypeJournal Article
Year of Publication2011
AuthorsBill, RM, Henderson, PJF, Iwata, S, Kunji, ERS, Michel, H, Neutze, R, Newstead, S, Poolman, B, Tate, CG, Vogel, H
JournalNat Biotechnol
Volume29
Issue4
Pagination335-40
Date Published2011 Apr
ISSN1546-1696
KeywordsCrystallization, Databases, Protein, Membrane Proteins, Protein Engineering, Protein Stability, Protein Structure, Tertiary, Protein Unfolding, Recombinant Proteins, X-Ray Diffraction
Abstract

After decades of slow progress, the pace of research on membrane protein structures is beginning to quicken thanks to various improvements in technology, including protein engineering and microfocus X-ray diffraction. Here we review these developments and, where possible, highlight generic new approaches to solving membrane protein structures based on recent technological advances. Rational approaches to overcoming the bottlenecks in the field are urgently required as membrane proteins, which typically comprise ~30% of the proteomes of organisms, are dramatically under-represented in the structural database of the Protein Data Bank.

DOI10.1038/nbt.1833
Alternate JournalNat. Biotechnol.
Citation Key10.1038/nbt.1833
PubMed ID21478852
Grant ListBB/G020043/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
G0900399 / / Medical Research Council / United Kingdom
MC_U105197215 / / Medical Research Council / United Kingdom
MC_U105663139 / / Medical Research Council / United Kingdom