Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids.

TitleHigher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids.
Publication TypeJournal Article
Year of Publication2005
AuthorsKlaus, SMJ, Kunji, ERS, Bozzo, GG, Noiriel, A, de la Garza, RDíaz, Basset, GJC, Ravanel, S, Rébeillé, F, Gregory, JF, Hanson, AD
JournalJ Biol Chem
Volume280
Issue46
Pagination38457-63
Date Published2005 Nov 18
ISSN0021-9258
KeywordsAminopterin, Animals, Arabidopsis, Arabidopsis Proteins, Biological Transport, Biopterin, Cell Membrane, Chlorophyll, Chloroplasts, Cloning, Molecular, Conserved Sequence, Cyanobacteria, Dicarboxylic Acid Transporters, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Folic Acid, Genome, Plant, Green Fluorescent Proteins, Leucovorin, Methotrexate, Models, Biological, Models, Chemical, Mutation, Peas, Peptides, Plastids, Protein Structure, Tertiary, Protein Transport, RNA, Messenger, Synechocystis, Trypanosoma
Abstract

Cyanobacterial and plant genomes encode proteins with some similarity to the folate and biopterin transporters of the trypanosomatid parasite Leishmania. The Synechocystis slr0642 gene product and its closest Arabidopsis homolog, the At2g32040 gene product, are representative examples. Both have 12 probable transmembrane domains, and the At2g32040 protein has a predicted chloroplast transit peptide. When expressed in Escherichia coli pabA pabB or folE, mutants, which are unable to produce or take up folates, the slr0642 protein and a modified At2g32040 protein (truncated and fused to the N terminus of slr0642) enabled growth on 5-formyltetrahydrofolate or folic acid but not on 5-formyltetrahydrofolate triglutamate, demonstrating that both proteins mediate folate monoglutamate transport. Both proteins also mediate transport of the antifolate analogs methotrexate and aminopterin, as evidenced by their ability to greatly increase the sensitivity of E. coli to these inhibitors. The full-length At2g32040 polypeptide was translocated into isolated pea chloroplasts and, when fused to green fluorescent protein, directed the passenger protein to the envelope of Arabidopsis chloroplasts in transient expression experiments. At2g32040 transcripts were present at similar levels in roots and aerial organs, indicating that the protein occurs in non-green plastids as well as chloroplasts. Insertional inactivation of At2g32040 significantly raised the total folate content of chloroplasts and lowered the proportion of 5-methyltetrahydrofolate but did not discernibly affect growth. These findings establish conservation of function among folate and biopterin transporter family proteins from three kingdoms of life.

DOI10.1074/jbc.M507432200
Alternate JournalJ. Biol. Chem.
Citation Key10.1074/jbc.M507432200
PubMed ID16162503
Grant ListR01 GM071382-01 / GM / NIGMS NIH HHS / United States