Conserved properties of hydrogenosomal and mitochondrial ADP/ATP carriers: a common origin for both organelles.

TitleConserved properties of hydrogenosomal and mitochondrial ADP/ATP carriers: a common origin for both organelles.
Publication TypeJournal Article
Year of Publication2002
Authorsvan der Giezen, M, Slotboom, DJan, Horner, DS, Dyal, PL, Harding, M, Xue, G-P, T Embley, M, Kunji, ERS
JournalEMBO J
Volume21
Issue4
Pagination572-9
Date Published2002 Feb 15
ISSN0261-4189
KeywordsAdenosine Diphosphate, Adenosine Triphosphate, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, Genetic Complementation Test, Humans, Hydrogen, Mitochondria, Mitochondrial ADP, ATP Translocases, Molecular Sequence Data, Phylogeny, Saccharomyces cerevisiae, Sequence Homology, Amino Acid
Abstract

Mitochondria are one of the hallmarks of eukaryotic cells, exporting ATP in exchange for cytosolic ADP using ADP/ATP carriers (AAC) located in the inner mitochondrial membrane. In contrast, several evolutionarily important anaerobic eukaryotes lack mitochondria but contain hydrogenosomes, peculiar organelles of controversial ancestry that also supply ATP but, like some fermentative bacteria, make molecular hydrogen in the process. We have now identified genes from two species of the hydrogenosome-containing fungus Neocallimastix that have three-fold sequence repeats and signature motifs that, along with phylogenetic analysis, identify them as AACs. When expressed in a mitochondrial AAC- deficient yeast strain, the hydrogenosomal protein was correctly targeted to the yeast mitochondria inner membrane and yielded mitochondria able to perform ADP/ATP exchange. Characteristic inhibitors of mitochondrial AACs blocked adenine nucleotide exchange by the Neocallimastix protein. Thus, our data demonstrate that fungal hydrogenosomes and yeast mitochondria use the same pathway for ADP/ATP exchange. These experiments provide some of the strongest evidence yet that yeast mitochondria and Neocallimastix hydrogenosomes are but two manifestations of the same fundamental organelle.

DOI10.1093/emboj/21.4.572
Alternate JournalEMBO J.
Citation Key10.1093/emboj/21.4.572
PubMed ID11847105
PubMed Central IDPMC125860
Grant List1U01 AI 48594-01 / AI / NIAID NIH HHS / United States