UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster.

TitleUCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster.
Publication TypeJournal Article
Year of Publication2014
AuthorsDa-Rè, C, De Pittà, C, Zordan, MA, Teza, G, Nestola, F, Zeviani, M, Costa, R, Bernardi, P
JournalEMBO Rep
Date Published2014 May
KeywordsAnimals, Cells, Cultured, Cold Temperature, Drosophila melanogaster, Drosophila Proteins, Enzyme Inhibitors, Gene Knockout Techniques, Guanosine Diphosphate, Larva, Membrane Transport Proteins, Mitochondria, Oligomycins, Oxygen Consumption, Palmitates, Respiration, Thermogenesis, Uncoupling Agents

Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F-ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva-to-adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein.

Alternate JournalEMBO Rep.
Citation Key10.1002/embr.201337972
PubMed ID24639557
PubMed Central IDPMC4210097
Grant ListGGP11011 / / Telethon / Italy
MC_UP_1002/1 / / Medical Research Council / United Kingdom