Characterization and expression of the mouse endonuclease G gene.

TitleCharacterization and expression of the mouse endonuclease G gene.
Publication TypeJournal Article
Year of Publication1997
AuthorsPrats, E, Noël, M, Létourneau, J, Tiranti, V, Vaqué, J, Debón, R, Zeviani, M, Cornudella, L, Ruiz-Carrillo, A
JournalDNA Cell Biol
Volume16
Issue9
Pagination1111-22
Date Published1997 Sep
ISSN1044-5498
KeywordsAlternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Cattle, Cloning, Molecular, DNA, Complementary, DNA, Mitochondrial, Endodeoxyribonucleases, Gene Dosage, Gene Expression Regulation, Enzymologic, Genes, Humans, Mice, Molecular Sequence Data, Organ Specificity, Promoter Regions, Genetic, Restriction Mapping, RNA, Messenger, Sequence Analysis, DNA
Abstract

Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.

DOI10.1089/dna.1997.16.1111
Alternate JournalDNA Cell Biol.
Citation Key10.1089/dna.1997.16.1111
PubMed ID9324313