Cloning of human and rat cDNAs encoding the mitochondrial single-stranded DNA-binding protein (SSB).

TitleCloning of human and rat cDNAs encoding the mitochondrial single-stranded DNA-binding protein (SSB).
Publication TypeJournal Article
Year of Publication1993
AuthorsTiranti, V, Rocchi, M, DiDonato, S, Zeviani, M
JournalGene
Volume126
Issue2
Pagination219-25
Date Published1993 Apr 30
ISSN0378-1119
KeywordsAmino Acid Sequence, Animals, Base Sequence, Biological Evolution, Blotting, Northern, Blotting, Southern, Chromosome Mapping, Chromosomes, Human, Pair 7, Cloning, Molecular, DNA, Mitochondrial, DNA, Single-Stranded, DNA-Binding Proteins, Humans, Mitochondria, Molecular Sequence Data, Rats, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid
Abstract

We have retro-transcribed and amplified by PCR the full-length cDNAs specifying the rat and human precursors of the single-stranded mitochondrial DNA (mtDNA)-binding protein (mtSSB). Each deduced sequence is composed of a 16-amino-acid (aa) N-terminal basic pre-sequence and a mature protein (132 aa in humans and 135 aa in the rat). The mature proteins are highly conserved among themselves and with the mtSSB from Xenopus laevis (Xl). Moreover, three regions of the protein are similar to corresponding domains of the SSB of Escherichia coli and to the E. coli F-sex factor SSB, indicating the existence of a broad class of DNA-binding proteins with structural and functional similarities both in prokaryotes and in prokaryote-derived organelles of higher organisms.

DOI10.1016/0378-1119(93)90370-i
Alternate JournalGene
Citation Key10.1016/0378-1119(93)90370-i
PubMed ID8482537
Grant List116 / / Telethon / Italy