Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase.

TitleSequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase.
Publication TypeJournal Article
Year of Publication1988
AuthorsZeviani, M, Sakoda, S, Sherbany, AA, Nakase, H, Rizzuto, R, Samitt, CE, DiMauro, S, Schon, EA
Date Published1988 May 15
KeywordsAmino Acid Sequence, Animals, Base Sequence, Cattle, Cloning, Molecular, DNA, DNA Restriction Enzymes, Electron Transport Complex IV, Genes, Humans, Macromolecular Substances, Mitochondria, Heart, Molecular Sequence Data, Nucleic Acid Hybridization, Nucleotide Mapping, Species Specificity

We have isolated a full-length human fetal muscle cDNA clone specifying the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme, cytochrome c oxidase (COX; EC, and a partial-length brain cDNA clone specifying the analogous bovine subunit. The two cDNAs are 85% identical at the nucleotide level. Similar to other proteins imported into mitochondria, the deduced human COX Vb protein contains a presequence, 31 amino acids long, rich in basic residues. We find no evidence for tissue-specific transcripts for subunit Vb of human COX, as Northern analysis of total RNA from human muscle, liver, and brain showed a single, identically sized transcript in each cell type, while partial-length cDNA clones isolated from human muscle and endothelial cell cDNA libraries were identical in sequence to the fetal muscle cDNA.

Alternate JournalGene
Citation Key10.1016/0378-1119(88)90411-8
PubMed ID2840351
Grant ListNS01035 / NS / NINDS NIH HHS / United States
NS11766 / NS / NINDS NIH HHS / United States