Cloning and expression of human nebulin cDNAs and assignment of the gene to chromosome 2q31-q32.

TitleCloning and expression of human nebulin cDNAs and assignment of the gene to chromosome 2q31-q32.
Publication TypeJournal Article
Year of Publication1988
AuthorsZeviani, M, Darras, BT, Rizzuto, R, Salviati, G, Betto, R, Bonilla, E, Miranda, AF, Du, J, Samitt, C, Dickson, G
JournalGenomics
Volume2
Issue3
Pagination249-56
Date Published1988 Apr
ISSN0888-7543
KeywordsAmino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 2, Cloning, Molecular, DNA, Gene Expression Regulation, Humans, Hybrid Cells, Molecular Sequence Data, Muscle Proteins, Rabbits
Abstract

We have isolated two nonoverlapping cDNAs encoding human nebulin, a muscle-specific protein. Northern hybridization analysis shows that nebulin is encoded by a huge message at least 25 kb in length. By hybridizing two nonoverlapping cDNAs to DNA isolated from rodent X human cell hybrids, we assign this presumably single-copy gene to human chromosome 2; sublocalization studies indicate that the nebulin gene is on the long arm of the chromosome, in the region 2q31-q32.

DOI10.1016/0888-7543(88)90009-2
Alternate JournalGenomics
Citation Key10.1016/0888-7543(88)90009-2
PubMed ID3397062
Grant ListGM32156 / GM / NIGMS NIH HHS / United States
NS11766 / NS / NINDS NIH HHS / United States