Isolation of a cDNA clone encoding subunit IV of human cytochrome c oxidase.

TitleIsolation of a cDNA clone encoding subunit IV of human cytochrome c oxidase.
Publication TypeJournal Article
Year of Publication1987
AuthorsZeviani, M, Nakagawa, M, Herbert, J, Lomax, MI, Grossman, LI, Sherbany, AA, Miranda, AF, DiMauro, S, Schon, EA
JournalGene
Volume55
Issue2-3
Pagination205-17
Date Published1987
ISSN0378-1119
KeywordsAmino Acid Sequence, Animals, Base Sequence, Cattle, DNA, DNA, Recombinant, Electron Transport Complex IV, Humans, Liver, Molecular Sequence Data, Nucleic Acid Hybridization, Organ Specificity, Phylogeny, Pseudogenes, RNA, Sequence Homology, Nucleic Acid
Abstract

We have isolated a full-length human liver cDNA clone specifying the nuclear-encoded subunit IV of the human mitochondrial respiratory chain enzyme, cytochrome c oxidase (COX; EC 1.9.3.1). The human cDNA clone is highly homologous to its bovine counterpart in the coding regions for both the mature polypeptide and the presequence, and the gene is evolving more slowly than that of any of the three mitochondrially encoded COX subunit genes. We find no preliminary evidence for tissue-specific isoforms of COX subunit IV, as Northern analysis of muscle, liver, and HeLa cell RNA shows an identically sized transcript in each cell type.

DOI10.1016/0378-1119(87)90281-2
Alternate JournalGene
Citation Key10.1016/0378-1119(87)90281-2
PubMed ID2444497
Grant ListNS01035 / NS / NINDS NIH HHS / United States
NS11766 / NS / NINDS NIH HHS / United States