Fibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress.

TitleFibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress.
Publication TypeJournal Article
Year of Publication2014
AuthorsPage, MM, Sinclair, A, Robb, EL, Stuart, JA, Withers, DJ, Selman, C
JournalAging Cell
Volume13
Issue5
Pagination962-4
Date Published2014 Oct
ISSN1474-9726
KeywordsAnimals, Female, Fibroblasts, Insulin Receptor Substrate Proteins, Male, Mice, Mice, Knockout, Oxidative Stress, Stress, Physiological
Abstract

Reduced signalling through the insulin/insulin-like growth factor-1 signalling (IIS) pathway is a highly conserved lifespan determinant in model organisms. The precise mechanism underlying the effects of the IIS on lifespan and health is currently unclear, although cellular stress resistance may be important. We have previously demonstrated that mice globally lacking insulin receptor substrate 1 (Irs1(-/-) ) are long-lived and enjoy a greater period of their life free from age-related pathology compared with wild-type (WT) controls. In this study, we show that primary dermal fibroblasts and primary myoblasts derived from Irs1(-/-) mice are no more resistant to a range of oxidant and nonoxidant chemical stressors than cells derived from WT mice.

DOI10.1111/acel.12255
Alternate JournalAging Cell
Citation Key10.1111/acel.12255
PubMed ID25059507
PubMed Central IDPMC4331740
Grant ListBB/H012850/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
BB/H020527/2 / / Biotechnology and Biological Sciences Research Council / United Kingdom
098565/Z/12/Z / / Wellcome Trust / United Kingdom
098565 / / Wellcome Trust / United Kingdom
MC_U120097114 / / Medical Research Council / United Kingdom
BB/H012850/2 / / Biotechnology and Biological Sciences Research Council / United Kingdom