The mitochondria targeted antioxidant MitoQ protects against fluoroquinolone-induced oxidative stress and mitochondrial membrane damage in human Achilles tendon cells.

TitleThe mitochondria targeted antioxidant MitoQ protects against fluoroquinolone-induced oxidative stress and mitochondrial membrane damage in human Achilles tendon cells.
Publication TypeJournal Article
Year of Publication2009
AuthorsLowes, DA, Wallace, C, Murphy, MP, Webster, NR, Galley, HF
JournalFree Radic Res
Volume43
Issue4
Pagination323-8
Date Published2009 Apr
ISSN1029-2470
KeywordsAchilles Tendon, Anti-Infective Agents, Antioxidants, Aza Compounds, Cell Survival, Cells, Cultured, Ciprofloxacin, Fluoroquinolones, Humans, Intracellular Membranes, Membrane Potential, Mitochondrial, Mitochondria, Organophosphorus Compounds, Oxidative Stress, Quinolines, Rupture, Tendinopathy, Ubiquinone
Abstract

Tendinitis and tendon rupture during treatment with fluoroquinolone antibiotics is thought to be mediated via oxidative stress. This study investigated whether ciprofloxacin and moxifloxacin cause oxidative stress and mitochondrial damage in cultured normal human Achilles' tendon cells and whether an antioxidant targeted to mitochondria (MitoQ) would protect against such damage better than a non-mitochondria targeted antioxidant. Human tendon cells from normal Achilles' tendons were exposed to 0-0.3 mM antibiotic for 24 h and 7 days in the presence of 1 microM MitoQ or an untargeted form, idebenone. Both moxifloxacin and ciprofloxacin resulted in up to a 3-fold increase in the rate of oxidation of dichlorodihydrofluorescein, a marker of general oxidative stress in tenocytes (p

DOI10.1080/10715760902736275
Alternate JournalFree Radic. Res.
Citation Key10.1080/10715760902736275
PubMed ID19235604
Grant ListMC_U105663142 / / Medical Research Council / United Kingdom