Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.

TitleMammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.
Publication TypeJournal Article
Year of Publication2010
AuthorsPohjoismäki, JLO, J Holmes, B, Wood, SR, Yang, M-Y, Yasukawa, T, Reyes, A, Bailey, LJ, Cluett, TJ, Goffart, S, Willcox, S, Rigby, RE, Jackson, AP, Spelbrink, JN, Griffith, JD, Crouch, RJ, Jacobs, HT, Holt, IJ
JournalJ Mol Biol
Volume397
Issue5
Pagination1144-55
Date Published2010 Apr 16
ISSN1089-8638
KeywordsAnimals, DNA, DNA Replication, DNA, Mitochondrial, Humans, Mammals, Nucleic Acid Conformation, Nucleic Acid Hybridization, RNA
Abstract

We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.

DOI10.1016/j.jmb.2010.02.029
Alternate JournalJ. Mol. Biol.
Citation Key10.1016/j.jmb.2010.02.029
PubMed ID20184890
PubMed Central IDPMC2857715
Grant ListMC_U105663140 / / Medical Research Council / United Kingdom
R01 ES013773 / ES / NIEHS NIH HHS / United States
GM31819 / GM / NIGMS NIH HHS / United States
R01 GM031819 / GM / NIGMS NIH HHS / United States
Z01 HD000068-36 / / Intramural NIH HHS / United States