An investigation of the effects of MitoQ on human peripheral mononuclear cells.

TitleAn investigation of the effects of MitoQ on human peripheral mononuclear cells.
Publication TypeJournal Article
Year of Publication2011
AuthorsMarthandan, S, Murphy, MP, Billett, E, Barnett, Y
JournalFree Radic Res
Volume45
Issue3
Pagination351-8
Date Published2011 Mar
ISSN1029-2470
KeywordsAdult, Aging, Antioxidants, DNA Damage, Glutathione, Humans, Hydrogen Peroxide, Leukocytes, Mononuclear, Membrane Potential, Mitochondrial, Middle Aged, Mitochondria, Organophosphorus Compounds, Oxidation-Reduction, Oxidative Stress, Ubiquinone
Abstract

MitoQ is a ubiquinone derivative targeted to mitochondria which is known to have both antioxidant and anti-apoptotic properties within mammalian cells. Previous research has suggested that the age-related increase in oxidative DNA damage in T lymphocytes might contribute to their functional decline with age. This paper describes the impact of mitoQ on unchallenged or oxidatively challenged ex vivo human peripheral blood mononuclear cells from healthy 25-30 or 55-60 year old volunteers. When cells were challenged with hydrogen peroxide (H(2)O(2)), following mitoQ treatment (0.1-1.0 μM), the ratio of reduced to oxidized forms of glutathione increased, the levels of oxidative DNA damage decreased and there was an increase in the mitochondrial membrane potential. Low levels of mitoQ (0.1 or 0.25 μM) had no impact on endogenous DNA damage, whilst higher levels (0.5 and 1.0 μM) of mitoQ significantly reduced endogenous levels of DNA damage. The results of this investigation suggest that mitoQ may have anti-immunosenescent potential.

DOI10.3109/10715762.2010.532497
Alternate JournalFree Radic. Res.
Citation Key10.3109/10715762.2010.532497
PubMed ID21047171
Grant ListMC_U105663142 / / Medical Research Council / United Kingdom