Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.

TitleSelective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.
Publication TypeJournal Article
Year of Publication2012
AuthorsChalmers, S, Caldwell, ST, Quin, C, Prime, TA, James, AM, Cairns, AG, Murphy, MP, McCarron, JG, Hartley, RC
JournalJ Am Chem Soc
Volume134
Issue2
Pagination758-61
Date Published2012 Jan 18
ISSN1520-5126
KeywordsAnimals, Cells, Cultured, Drug Delivery Systems, Mitochondria, Molecular Structure, Myocytes, Smooth Muscle, Organometallic Compounds, Organophosphorus Compounds, Ultraviolet Rays
Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

DOI10.1021/ja2077922
Alternate JournalJ. Am. Chem. Soc.
Citation Key10.1021/ja2077922
PubMed ID22239373
PubMed Central IDPMC3260739
Grant ListMC_U105663142 / / Medical Research Council / United Kingdom
092292/B/10/Z / / Wellcome Trust / United Kingdom
092292/Z/10/Z / / Wellcome Trust / United Kingdom
/ / Biotechnology and Biological Sciences Research Council / United Kingdom