Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.

TitleResolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.
Publication TypeJournal Article
Year of Publication2012
AuthorsPung, YFen, Rocic, P, Murphy, MP, Smith, RAJ, Hafemeister, J, Ohanyan, V, Guarini, G, Yin, L, Chilian, WM
JournalArterioscler Thromb Vasc Biol
Volume32
Issue2
Pagination325-34
Date Published2012 Feb
ISSN1524-4636
KeywordsAnimals, Antioxidants, Collateral Circulation, Coronary Vessels, Disease Models, Animal, Lipid Peroxidation, Lipid Peroxides, Male, Metabolic Syndrome, Mitochondria, Heart, Mitochondrial Proteins, Obesity, Organophosphorus Compounds, Oxidative Stress, Piperidines, Rats, Rats, Zucker, Reactive Oxygen Species, Ubiquinone
Abstract

OBJECTIVE: We have previously found abrogated ischemia-induced coronary collateral growth in Zucker obese fatty (ZOF) rats compared with Zucker lean (ZLN) rats. Because ZOF rats have structural abnormalities in their mitochondria suggesting dysfunction and also show increased production of O(2), we hypothesized that mitochondrial dysfunction caused by oxidative stress impairs coronary collateral growth in ZOF.METHODS AND RESULTS: Increased levels of reactive oxygen species were observed in aortic endothelium and smooth muscle cells in ZOF rats compared with ZLN rats. Reactive oxygen species levels were decreased by the mitochondria-targeted antioxidants MitoQuinone (MQ) and MitoTempol (MT) as assessed by MitoSox Red and dihydroethidine staining. Lipid peroxides (a marker of oxidized lipids) were increased in ZOF by ≈47% compared with ZLN rats. The elevation in oxidative stress was accompanied by increased antioxidant enzymes, except glutathione peroxidase-1, and by increased uncoupling protein-2 in ZOF versus ZLN rats. In addition, elevated respiration rates were also observed in the obese compared with lean rats. Administration of MQ significantly normalized the metabolic profiles and reduced lipid peroxides in ZOF rats to the same level observed in lean rats. The protective effect of MQ also suppressed the induction of uncoupling protein-2 in the obese rats. Resolution of mitochondrial oxidative stress by MQ or MT restored coronary collateral growth to the same magnitude observed in ZLN rats in response to repetitive ischemia.CONCLUSIONS: We conclude that mitochondrial oxidative stress and dysfunction play a key role in disrupting coronary collateral growth in obesity and the metabolic syndrome, and elimination of the mitochondrial oxidative stress with MQ or MT rescues collateral growth.

DOI10.1161/ATVBAHA.111.241802
Alternate JournalArterioscler. Thromb. Vasc. Biol.
Citation Key10.1161/ATVBAHA.111.241802
PubMed ID22155454
PubMed Central IDPMC4013346
Grant ListR01 HL032788-22 / HL / NHLBI NIH HHS / United States
RC1 HL100828 / HL / NHLBI NIH HHS / United States
RC1HL100828 / HL / NHLBI NIH HHS / United States
MC_U105663142 / / Medical Research Council / United Kingdom
HL83366 / HL / NHLBI NIH HHS / United States
R01 HL032788 / HL / NHLBI NIH HHS / United States
R01 HL083366 / HL / NHLBI NIH HHS / United States
RC1 HL100828-02 / HL / NHLBI NIH HHS / United States
R01 HL083366-04 / HL / NHLBI NIH HHS / United States
HL32788 / HL / NHLBI NIH HHS / United States