Quantitative analysis using gas chromatography/combustion/isotope ratio mass spectrometry and standard addition of intrinsically labelled standards (SAIL)--application to isoflavones in foods.

TitleQuantitative analysis using gas chromatography/combustion/isotope ratio mass spectrometry and standard addition of intrinsically labelled standards (SAIL)--application to isoflavones in foods.
Publication TypeJournal Article
Year of Publication2002
AuthorsBluck, LJC, Jones, KS, Thomas, J, Liggins, J, Harding, M, Bingham, SA, W Coward, A
JournalRapid Commun Mass Spectrom
Volume16
Issue24
Pagination2249-54
Date Published2002
ISSN0951-4198
KeywordsCarbon Isotopes, Estrogens, Non-Steroidal, Flour, Food Analysis, Gas Chromatography-Mass Spectrometry, Genistein, Isoflavones, Phytoestrogens, Plant Preparations, Reference Values, Soybeans
Abstract

We have investigated a novel application of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) for the quantitative analysis of the isoflavones in food matrices. Previous methods have been hampered by the lack of analytical standards to introduce early enough in the extraction procedure to allow compensations for losses at all stages of the procedure. In this work we have produced standard materials that can be added at the initial extraction, by intrinsically labelling soya plants by growing them in an atmosphere enriched in the stable isotope of carbon in CO(2). On analysis these plants were shown to contain phytoestrogens at a high (up to 20%) level of enrichment. The dried plant material has been used to estimate the isoflavone concentrations of a set of spiked flours. For daidzein the methodology was shown to produce results comparable to those achieved by GC/MS techniques. The method was less successful for genistein, possibly due to the greater fragility of this compound under the conditions required for the analysis.

DOI10.1002/rcm.850
Alternate JournalRapid Commun. Mass Spectrom.
Citation Key10.1002/rcm.850
PubMed ID12478567