Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool.

TitleAnalysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool.
Publication TypeJournal Article
Year of Publication2002
AuthorsR Davies, J, Freeman, A, Morris, LS, Bingham, S, Dilworth, S, Scott, I, Laskey, RA, Miller, R, Coleman, N
JournalLancet
Volume359
Issue9321
Pagination1917-9
Date Published2002 Jun 01
ISSN0140-6736
KeywordsAdenocarcinoma, Analysis of Variance, Biomarkers, Tumor, Case-Control Studies, Colorectal Neoplasms, Feces, Humans, Minichromosome Maintenance Complex Component 2, Nuclear Proteins, Reproducibility of Results
Abstract

Colorectal cancer is a common disease, and more reliable screening methods are needed for early detection. We aim to develop a non-invasive, stool-based assay that can identify colorectal cancer by detection of minichromosome maintenance protein 2 (MCM2) expression in colonocytes retrieved from the faecal surface. We devised a cell line model to investigate methods and conditions for optimum colonocyte retrieval. In our clinical evaluation study, MCM2-positive cells were retrieved from 37 of 40 patients with symptomatic colorectal cancer, but from none of 25 healthy control individuals. These results suggest that immunocytochemical analysis of retrieved colonocytes might enable accurate detection of colorectal cancer in stool.

DOI10.1016/S0140-6736(02)08739-1
Alternate JournalLancet
Citation Key10.1016/S0140-6736(02)08739-1
PubMed ID12057556