Detection and interpretation of redox potential optima in the catalytic activity of enzymes.

TitleDetection and interpretation of redox potential optima in the catalytic activity of enzymes.
Publication TypeJournal Article
Year of Publication2002
AuthorsElliott, SJ, Léger, C, Pershad, HR, Hirst, J, Heffron, K, Ginet, N, Blasco, F, Rothery, RA, Weiner, JH, Armstrong, FA
JournalBiochim Biophys Acta
Volume1555
Issue1-3
Pagination54-9
Date Published2002 Sep 10
ISSN0006-3002
KeywordsAnimals, Catalysis, Cattle, Electrochemistry, Electron Transport, Escherichia coli, Hydrogen-Ion Concentration, Iron-Sulfur Proteins, Mitochondria, Nitrate Reductase, Nitrate Reductases, Oxidation-Reduction, Oxidoreductases, Succinate Dehydrogenase
Abstract

It is no surprise that the catalytic activity of electron-transport enzymes may be optimised at certain electrochemical potentials in ways that are analogous to observations of pH-rate optima. This property is observed clearly in experiments in which an enzyme is adsorbed on an electrode surface which can supply or receive electrons rapidly and in a highly controlled manner. In such a way, the rate of catalysis can be measured accurately as a function of the potential (driving force) that is applied. In this paper, we draw attention to a few examples in which this property has been observed in enzymes that are associated with membrane-bound respiratory chains, and we discuss its possible origins and implications for in vivo regulation.

DOI10.1016/s0005-2728(02)00254-2
Alternate JournalBiochim. Biophys. Acta
Citation Key10.1016/s0005-2728(02)00254-2
PubMed ID12206891