Detection of malondialdehyde DNA adducts in human colorectal mucosa: relationship with diet and the presence of adenomas.

TitleDetection of malondialdehyde DNA adducts in human colorectal mucosa: relationship with diet and the presence of adenomas.
Publication TypeJournal Article
Year of Publication2002
AuthorsLeuratti, C, Watson, MA, Deag, EJ, Welch, A, Singh, R, Gottschalg, E, Marnett, LJ, Atkin, W, Day, NE, Shuker, DEG, Bingham, SA
JournalCancer Epidemiol Biomarkers Prev
Volume11
Issue3
Pagination267-73
Date Published2002 Mar
ISSN1055-9965
KeywordsAdenoma, Colon, Colorectal Neoplasms, Deoxyguanosine, DNA Adducts, Female, Food Habits, Humans, Immunoblotting, Intestinal Mucosa, Male, Middle Aged, Predictive Value of Tests, Prospective Studies, Rectum, Risk Factors, Tumor Markers, Biological
Abstract

Colorectal biopsies from normal mucosa of participants in the United Kingdom Flexible Sigmoidoscopy Trial and European Prospective Investigation on Cancer (EPIC; n = 162) were analyzed for the presence of malondialdehyde-deoxyguanosine (M(1)-dG), a DNA adduct derived from lipid peroxidation. The aim was to investigate whether dietary factors can modulate M(1)-dG levels and whether M(1)-dG in normal mucosa is a risk factor for colorectal adenomas. Samples were analyzed using a sensitive immunoblot blot assay. This study has shown for the first time that M(1)-dG is present in human colorectal tissue. M(1)-dG levels ranged from undetectable (n = 13) to 12.23 per 10(7) total bases. Mean levels were 4.3 +/- 3 and 4.6 +/- 2.9 per 10(7) total bases in men and women, respectively. In men, there were positive associations of adduct levels with height and age, and inverse associations with body mass index. Legumes, fruit, salad, and whole meal bread were inversely associated with M(1)-dG adducts, whereas consumption of offal, white meat, beer, and alcohol were positively associated with elevated levels. In women, there was an inverse association of the adduct with the ratio of polyunsaturated:saturated fatty acids (P = 0.019) and a weak positive correlation with saturated fat (P

Alternate JournalCancer Epidemiol. Biomarkers Prev.
Citation Key1943
PubMed ID11895876
Grant ListCA-77839 / CA / NCI NIH HHS / United States