Nonsteroidal antiinflammatory drugs and a selective cyclooxygenase 2 inhibitor uncouple mitochondria in intact cells.

TitleNonsteroidal antiinflammatory drugs and a selective cyclooxygenase 2 inhibitor uncouple mitochondria in intact cells.
Publication TypeJournal Article
Year of Publication2003
AuthorsKrause, MM, Brand, MD, Krauss, S, Meisel, C, Vergin, H, Burmester, G-R, Buttgereit, F
JournalArthritis Rheum
Volume48
Issue5
Pagination1438-44
Date Published2003 May
ISSN0004-3591
KeywordsAnimals, Anti-Inflammatory Agents, Non-Steroidal, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Cyclooxygenase Inhibitors, Diclofenac, Dose-Response Relationship, Drug, Female, Indomethacin, Intracellular Membranes, Isoenzymes, Membrane Potentials, Mitochondria, Liver, Oxidative Phosphorylation, Oxygen Consumption, Prostaglandin-Endoperoxide Synthases, Pyrazoles, Rats, Rats, Wistar, Sulfonamides, Thymus Gland
Abstract

OBJECTIVE: Uncoupling of isolated mitochondria by nonsteroidal antiinflammatory drugs (NSAIDs) has been considered relevant to the development of gastrointestinal (GI) side effects. We investigated the occurrence of NSAID-induced uncoupling of mitochondria in intact cells (rat thymocytes) compared with the effects of a selective cyclooxygenase 2 (COX-2) inhibitor.METHODS: Oxygen consumption and mitochondrial membrane potential were simultaneously measured amperometrically and by distribution of radioactive tracer molecules, respectively, in the presence and absence of pharmacologically relevant concentrations of the NSAIDs indomethacin and diclofenac and the selective COX-2 inhibitor SC-236. Analysis of data by a technique related to top-down elasticity analysis permitted assessment of the influence of these compounds on individual components of cellular energy metabolism.RESULTS: Indomethacin, diclofenac, and SC-236 increased proton leak in isolated mitochondria. Both diclofenac and SC-236 significantly stimulated proton leak in intact cells and simultaneously inhibited substrate oxidation and ATP turnover. Oxygen consumption rates of isolated cells remained unchanged over a wide concentration range of the drugs, despite significant effects on subsystems of cellular energy metabolism.CONCLUSION: NSAIDs and selective COX-2 inhibitors have significant and equally directed effects on cellular energy metabolism. They both uncouple mitochondrial respiration and inhibit substrate oxidation and ATP turnover. However, the topical effect and selective COX-2 inhibition may not be sufficient to cause NSAID-like damage to the GI tract.

DOI10.1002/art.10969
Alternate JournalArthritis Rheum.
Citation Key10.1002/art.10969
PubMed ID12746918