Using mitochondria-targeted molecules to study mitochondrial radical production and its consequences.

TitleUsing mitochondria-targeted molecules to study mitochondrial radical production and its consequences.
Publication TypeJournal Article
Year of Publication2003
AuthorsSmith, RAJ, Kelso, GF, Blaikie, FH, Porteous, CM, Ledgerwood, EC, Hughes, G, James, AM, Ross, MF, Asin-Cayuela, J, Cochemé, HM, Filipovska, A, Murphy, MP
JournalBiochem Soc Trans
Volume31
IssuePt 6
Pagination1295-9
Date Published2003 Dec
ISSN0300-5127
KeywordsAnimals, Antioxidants, Mitochondria, Molecular Probes, Reactive Oxygen Species, Sulfhydryl Compounds
Abstract

The production of ROS (reactive oxygen species) by the mitochondrial respiratory chain contributes to a range of pathologies, including neurodegenerative diseases, ischaemia/reperfusion injury and aging. There are also indications that mitochondrial ROS production plays a role in damage response and signal transduction pathways. To unravel the role of mitochondrial ROS production in these processes, we have developed a range of mitochondria-targeted probe molecules. Covalent attachment of a lipophilic cation leads to their accumulation into mitochondria, driven by the membrane potential. Molecules developed so far include antioxidants designed to intercept mitochondrial ROS and reagents that specifically label mitochondrial thiol proteins. Here we outline how mitochondrial ROS formation and its consequences can be investigated using these probes.

DOI10.1042/bst0311295
Alternate JournalBiochem. Soc. Trans.
Citation Key10.1042/bst0311295
PubMed ID14641046