Mitochondria-targeted redox probes as tools in the study of oxidative damage and ageing.

TitleMitochondria-targeted redox probes as tools in the study of oxidative damage and ageing.
Publication TypeJournal Article
Year of Publication2005
AuthorsJames, AM, Cochemé, HM, Murphy, MP
JournalMech Ageing Dev
Volume126
Issue9
Pagination982-6
Date Published2005 Sep
ISSN0047-6374
KeywordsAging, Animals, Antioxidants, Cations, Electrons, Humans, Mitochondria, Models, Biological, Molecular Probes, Oxidation-Reduction, Oxidative Stress, Oxygen, Phosphorylation, Reactive Oxygen Species, Ubiquinone
Abstract

Mitochondrial reactive oxygen species (ROS) and oxidative damage are associated with a range of age-related human pathologies. It is also likely that mitochondrial ROS generation is a factor in stress response and signal transduction pathways. However, current methods for measuring and influencing mitochondrial ROS production in vivo often lack the desired specificity. To help elucidate the potential role of mitochondrial ROS production in ageing, we have developed a range of mitochondria-targeted ROS probes that may be useful in vivo. This was achieved by covalently attaching a lipophilic cation to a ROS-reactive moiety causing its membrane potential-dependent accumulation within mitochondria. Mitochondria-targeted molecules developed so far include antioxidants that detoxify mitochondrial ROS, probes that react with mitochondrial ROS, and reagents that specifically label mitochondrial protein thiols. Here, we outline how the formation and consequences of mitochondrial ROS production can be investigated using these probes.

DOI10.1016/j.mad.2005.03.026
Alternate JournalMech. Ageing Dev.
Citation Key10.1016/j.mad.2005.03.026
PubMed ID15923020