The effect of dietary restriction on mitochondrial protein density and flight muscle mitochondrial morphology in Drosophila.

TitleThe effect of dietary restriction on mitochondrial protein density and flight muscle mitochondrial morphology in Drosophila.
Publication TypeJournal Article
Year of Publication2006
AuthorsMagwere, T, Goodall, S, Skepper, J, Mair, W, Brand, MD, Partridge, L
JournalJ Gerontol A Biol Sci Med Sci
Volume61
Issue1
Pagination36-47
Date Published2006 Jan
ISSN1079-5006
KeywordsAge Factors, Aging, Animals, Caloric Restriction, Drosophila melanogaster, Microscopy, Electron, Mitochondria, Muscle, Mitochondrial Proteins, Muscle, Skeletal
Abstract

Dietary restriction (DR) extends life span in diverse organisms and may do so by attenuating production of mitochondrial reactive oxygen species (ROS). However, measurements of ROS production from isolated mitochondria of organisms subjected to DR have produced inconsistent results. In the fruit fly Drosophila, DR does not reduce production of ROS from isolated mitochondria. In this study, we used Drosophila to test whether DR lowered mitochondrial density. We assessed mitochondrial densities of flies on DR and Control diets using (a) the activities of mitochondrial enzymes and (b) electron microscopy. Both methods showed no overall effect of DR on mitochondrial density; however, mitochondrial enzyme activities and morphology differed significantly between DR and Control flies. We concluded that life-span extension by DR in Drosophila is not mediated through a reduction in mitochondrial density. If DR in Drosophila extends life span by reducing ROS production, then it does so through mechanisms that operate only in vivo.

Alternate JournalJ. Gerontol. A Biol. Sci. Med. Sci.
Citation Key2067
PubMed ID16456193
Grant ListMC_U105663137 / / Medical Research Council / United Kingdom
SF19106 / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom