An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.

TitleAn iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
Publication TypeJournal Article
Year of Publication2007
AuthorsKlinge, S, Hirst, J, Maman, JD, Krude, T, Pellegrini, L
JournalNat Struct Mol Biol
Volume14
Issue9
Pagination875-7
Date Published2007 Sep
ISSN1545-9993
KeywordsDNA Primase, Electron Spin Resonance Spectroscopy, Iron-Sulfur Proteins, Protein Binding, RNA
Abstract

Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.

DOI10.1038/nsmb1288
Alternate JournalNat. Struct. Mol. Biol.
Citation Key10.1038/nsmb1288
PubMed ID17704817
PubMed Central IDPMC2268749
Grant List071102 / / Wellcome Trust / United Kingdom
MC_U105663141 / / Medical Research Council / United Kingdom