The apoptotic regulator Nrz controls cytoskeletal dynamics via the regulation of Ca2+ trafficking in the zebrafish blastula.

TitleThe apoptotic regulator Nrz controls cytoskeletal dynamics via the regulation of Ca2+ trafficking in the zebrafish blastula.
Publication TypeJournal Article
Year of Publication2011
AuthorsPopgeorgiev, N, Bonneau, B, Ferri, KF, Prudent, J, Thibaut, J, Gillet, G
JournalDev Cell
Volume20
Issue5
Pagination663-76
Date Published2011 May 17
ISSN1878-1551
KeywordsAnimals, Apoptosis, Blastula, Calcium, Cytoskeleton, Endoplasmic Reticulum, HeLa Cells, Humans, Proto-Oncogene Proteins, Zebrafish, Zebrafish Proteins
Abstract

Bcl-2 family members are key regulators of apoptosis. Their involvement in other cellular processes has been so far overlooked. We have studied the role of the Bcl-2 homolog Nrz in the developing zebrafish. Nrz was found to be localized to the yolk syncytial layer, a region containing numerous mitochondria and ER membranes. Nrz knockdown resulted in developmental arrest before gastrulation, due to free Ca(2+) increase in the yolk cell, activating myosin light chain kinase, which led to premature contraction of actin-myosin cables in the margin and separation of the blastomeres from the yolk cell. In the yolk syncytial layer, Nrz appears to prevent the release of Ca(2+) from the endoplasmic reticulum by directly interacting with the IP3R1 Ca(2+) channel. Thus, the Bcl-2 family may participate in early development, not only by controlling apoptosis but also by acting on cytoskeletal dynamics and cell movements via Ca(2+) fluxes inside the embryo.

DOI10.1016/j.devcel.2011.03.016
Alternate JournalDev. Cell
Citation Key10.1016/j.devcel.2011.03.016
PubMed ID21571223