Membrane Protein Production in Lactococcus lactis for Functional Studies.

TitleMembrane Protein Production in Lactococcus lactis for Functional Studies.
Publication TypeJournal Article
Year of Publication2016
AuthorsSeigneurin-Berny, D, King, MS, Sautron, E, Moyet, L, Catty, P, André, F, Rolland, N, Kunji, ERS, Frelet-Barrand, A
JournalMethods Mol Biol
Volume1432
Pagination79-101
Date Published2016
ISSN1940-6029
KeywordsBacterial Proteins, Gene Expression, Genetic Vectors, Lactococcus lactis, Membrane Proteins, Protein Engineering, Recombinant Proteins
Abstract

Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.

DOI10.1007/978-1-4939-3637-3_6
Alternate JournalMethods Mol. Biol.
Citation Key10.1007/978-1-4939-3637-3_6
PubMed ID27485331
Grant ListMC_U105663139 / / Medical Research Council / United Kingdom