The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B.

TitleThe structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B.
Publication TypeJournal Article
Year of Publication1996
Authorsvan Raaij, MJ, Abrahams, JP, Leslie, AG, Walker, JE
JournalProc Natl Acad Sci U S A
Volume93
Issue14
Pagination6913-7
Date Published1996 Jul 09
ISSN0027-8424
KeywordsAdenylyl Imidodiphosphate, Animals, Arginine, Aurovertins, Binding Sites, Cattle, Crystallography, X-Ray, Enzyme Inhibitors, Glutamic Acid, Macromolecular Substances, Models, Molecular, Molecular Structure, Myocardium, Protein Structure, Secondary, Proton-Translocating ATPases
Abstract

In the structure of bovine mitochondrial F1-ATPase that was previously determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have different conformations and nucleotide occupancies. Adenylyl-imidodiphosphate is bound to one beta-subunit (betaTP), ADP is bound to the second (betaDP), and no nucleotide is bound to the third (betaE). Here we show that the uncompetitive inhibitor aurovertin B binds to bovine F1 at two equivalent sites in betaTP and betaE, in a cleft between the nucleotide binding and C-terminal domains. In betaDP, the aurovertin B pocket is incomplete and is inaccessible to the inhibitor. The aurovertin B bound to betaTP interacts with alpha-Glu399 in the adjacent alphaTP subunit, whereas the aurovertin B bound to betaE is too distant from alphaE to make an equivalent interaction. Both sites encompass betaArg-412, which was shown by mutational studies to be involved in binding aurovertin. Except for minor changes around the aurovertin pockets, the structure of bovine F1-ATPase is the same as determined previously. Aurovertin B appears to act by preventing closure of the catalytic interfaces, which is essential for a catalytic mechanism involving cyclic interconversion of catalytic sites.

DOI10.1073/pnas.93.14.6913
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
Citation Key10.1073/pnas.93.14.6913
PubMed ID8692918
PubMed Central IDPMC38908