Complete amino acid sequence of the ADP/ATP carrier from beef heart mitochondria.

TitleComplete amino acid sequence of the ADP/ATP carrier from beef heart mitochondria.
Publication TypeJournal Article
Year of Publication1982
AuthorsAquila, H, Misra, D, Eulitz, M, Klingenberg, M
JournalHoppe Seylers Z Physiol Chem
Volume363
Issue3
Pagination345-9
Date Published1982 Mar
ISSN0018-4888
KeywordsAmino Acid Sequence, Animals, Cattle, Cyanogen Bromide, Mitochondria, Heart, Molecular Weight, Peptide Fragments
Abstract

The complete primary structure of the ADP/ATP carrier from beef heart mitochondria is described. Cyanogen bromide cleaves the protein into a long, N-terminally blocked fragment with Mr 22,000 (CB1) and several small peptides. The primary information was derived from liquid-phase sequencing of tryptic peptides obtained from the maleylated carrier protein and the citraconylated CB1 fragment, as well as from cleavage products with Staphylococcus aureus protease. The multitude of thermolysinolytic, tryptic, chymotryptic and peptic peptides was sequenced by manual methods. They rendered overlaps and further supported already known partial sequences. Also, the bridge between the published acidolytic C-terminal fragment A2 was thus obtained.

Alternate JournalHoppe-Seyler's Z. Physiol. Chem.
Citation KeyPMID7076130
PubMed ID7076130