DAPIT, a novel protein down-regulated in insulin-sensitive tissues in streptozotocin-induced diabetes.

TitleDAPIT, a novel protein down-regulated in insulin-sensitive tissues in streptozotocin-induced diabetes.
Publication TypeJournal Article
Year of Publication2001
AuthorsPäivärinne, H, Kainulainen, H
JournalActa Diabetol
Volume38
Issue2
Pagination83-6
Date Published2001
ISSN0940-5429
KeywordsAmino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Diabetes Mellitus, Experimental, DNA, Complementary, Gene Expression Regulation, Insulin, Male, Membrane Proteins, Molecular Sequence Data, Muscle, Skeletal, Myocardium, Open Reading Frames, Organ Specificity, Polymerase Chain Reaction, Rats, Rats, Sprague-Dawley, RNA, Messenger, Sequence Analysis, DNA
Abstract

We have identified a novel gene product differentially expressed in skeletal muscle in a rat diabetes model induced by streptozotocin. Northern blot analysis showed expression in all studied tissues and a marked down-regulation in insulin-sensitive tissues (skeletal muscle, myocardium) but not in insulin-insensitive brain. cDNA sequence analysis revealed an open reading frame (ORF) of 58 aminoacids, containing one putative transmembrane domain. We designated this protein DAPIT (diabetes-associated protein in insulin-sensitive tissues). Database searches discovered several human, rat, mouse, rabbit, bovine, equine, porcine, Fugu and Xenopus ESTs with significant homology in the ORF. The aminoacid sequence of DAPIT is similar to that of a putative protein of Drosophila melanogaster and to a cAMP-generating peptide isolated from the flesh fly Neobellieria bullata. We propose that DAPIT is a novel protein that is highly conserved in vertebrates and insects.

DOI10.1007/s005920170018
Alternate JournalActa Diabetol
Citation Key10.1007/s005920170018
PubMed ID11757806