Sequences of members of the human gene family for the c subunit of mitochondrial ATP synthase.

TitleSequences of members of the human gene family for the c subunit of mitochondrial ATP synthase.
Publication TypeJournal Article
Year of Publication1993
AuthorsDyer, MR, Walker, JE
JournalBiochem J
Volume293 ( Pt 1)
Pagination51-64
Date Published1993 Jul 01
ISSN0264-6021
KeywordsAnimals, Base Sequence, Cattle, Cloning, Molecular, Gene Expression, Genomic Library, Humans, Mitochondria, Molecular Sequence Data, Nucleic Acid Hybridization, Placenta, Proton-Translocating ATPases, Pseudogenes
Abstract

Subunit c is an intrinsic membrane component of ATP synthase, and in mammals it is encoded by two expressed nuclear genes, P1 and P2. Both genes encode the same mature c subunit, but the mitochondrial import pre-sequences in the precursors of subunit c are different. The DNA sequences of the human P1 and P2 genes are described. They occupy about 3.0 and 10.9 kb respectively of the human genome, and both genes are split into five exons. The human genome also contains about 14 related spliced pseudogenes, and the sequence of one such pseudogene related to P2 is described. Sequences flanking the 5' ends of the human P1 and P2 coding sequences each contain a CpG-rich island. Potential promoter elements (TATA and CCAAT boxes) are present in the 5' sequences of the P1 gene, but not that of P2, although there is no direct experimental evidence to show the involvement of these sequences in transcription of the genes.

DOI10.1042/bj2930051
Alternate JournalBiochem. J.
Citation Key10.1042/bj2930051
PubMed ID8328972
PubMed Central IDPMC1134319