Respiratory Complex I in Bos taurus and Paracoccus denitrificans Pumps Four Protons across the Membrane for Every NADH Oxidized.

TitleRespiratory Complex I in Bos taurus and Paracoccus denitrificans Pumps Four Protons across the Membrane for Every NADH Oxidized.
Publication TypeJournal Article
Year of Publication2017
AuthorsJ Y Jones, A, Blaza, JN, Varghese, F, Hirst, J
JournalJ Biol Chem
Volume292
Issue12
Pagination4987-4995
Date Published2017 Mar 24
ISSN1083-351X
KeywordsAdenosine Triphosphate, Animals, Cattle, Electron Transport, Electron Transport Complex I, Mitochondria, NAD, Oxidation-Reduction, Oxidative Phosphorylation, Paracoccus denitrificans, Proton-Motive Force, Protons
Abstract

Respiratory complex I couples electron transfer between NADH and ubiquinone to proton translocation across an energy-transducing membrane to support the proton-motive force that drives ATP synthesis. The proton-pumping stoichiometry of complex I (i.e. the number of protons pumped for each two electrons transferred) underpins all mechanistic proposals. However, it remains controversial and has not been determined for any of the bacterial enzymes that are exploited as model systems for the mammalian enzyme. Here, we describe a simple method for determining the proton-pumping stoichiometry of complex I in inverted membrane vesicles under steady-state ADP-phosphorylating conditions. Our method exploits the rate of ATP synthesis, driven by oxidation of NADH or succinate with different sections of the respiratory chain engaged in catalysis as a proxy for the rate of proton translocation and determines the stoichiometry of complex I by reference to the known stoichiometries of complexes III and IV. Using vesicles prepared from mammalian mitochondria (from Bos taurus) and from the bacterium Paracoccus denitrificans, we show that four protons are pumped for every two electrons transferred in both cases. By confirming the four-proton stoichiometry for mammalian complex I and, for the first time, demonstrating the same value for a bacterial complex, we establish the utility of P. denitrificans complex I as a model system for the mammalian enzyme. P. denitrificans is the first system described in which mutagenesis in any complex I core subunit may be combined with quantitative proton-pumping measurements for mechanistic studies.

DOI10.1074/jbc.M116.771899
Alternate JournalJ. Biol. Chem.
Citation Key10.1074/jbc.M116.771899
PubMed ID28174301
PubMed Central IDPMC5377811
Grant ListMC_U105663141 / / Medical Research Council / United Kingdom