Human mitochondrial ribosomes can switch structural tRNAs - but when and why?

TitleHuman mitochondrial ribosomes can switch structural tRNAs - but when and why?
Publication TypeJournal Article
Year of Publication2017
AuthorsChrzanowska-Lightowlers, Z, Rorbach, J, Minczuk, MA
JournalRNA Biol
Date Published2017 Aug 08
ISSN1555-8584
Abstract

High resolution cryoEM of mammalian mitoribosomes revealed the unexpected presence of mitochondrially encoded tRNA as a structural component of mitochondrial large ribosomal subunit (mt-LSU). Our previously published data identified that only mitochondrial (mt-) tRNA(Phe) and mt-tRNA(Val) can be incorporated into mammalian mt-LSU and within an organism there is no evidence of tissue specific variation. When mt-tRNA(Val) is limiting, human mitoribosomes can integrate mt-tRNA(Phe) instead to generate a translationally competent monosome. Here we discuss the possible reasons for and consequences of the observed plasticity of the structural mt-tRNA integration. We also indicate potential direction for further research that could help our understanding of the mechanistic and evolutionary aspects of this unprecedented system.

DOI10.1080/15476286.2017.1356551
Alternate JournalRNA Biol
Citation Key10.1080/15476286.2017.1356551
PubMed ID28786741