|Title||MitoNeoD: A Mitochondria-Targeted Superoxide Probe.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Shchepinova, MM, Cairns, AG, Prime, TA, Logan, A, James, AM, Hall, AR, Vidoni, S, Arndt, S, Caldwell, ST, Prag, HA, Pell, VR, Krieg, T, Mulvey, JF, Yadav, P, Cobley, JN, Bright, TP, Senn, HM, Anderson, RF, Murphy, MP, Hartley, RC|
|Journal||Cell Chem Biol|
|Date Published||2017 Aug 24|
Mitochondrial superoxide (O2(⋅-)) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2(⋅-), but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed a dual-purpose mitochondrial O2(⋅-) probe, MitoNeoD, which can assess O2(⋅-) changes in vivo by mass spectrometry and in vitro by fluorescence. MitoNeoD comprises a O2(⋅-)-sensitive reduced phenanthridinium moiety modified to prevent DNA intercalation, as well as a carbon-deuterium bond to enhance its selectivity for O2(⋅-) over non-specific oxidation, and a triphenylphosphonium lipophilic cation moiety leading to the rapid accumulation within mitochondria. We demonstrated that MitoNeoD was a versatile and robust probe to assess changes in mitochondrial O2(⋅-) from isolated mitochondria to animal models, thus offering a way to examine the many roles of mitochondrial O2(⋅-) production in health and disease.
|Alternate Journal||Cell Chem Biol|