ClickIn: a flexible protocol for quantifying mitochondrial uptake of nucleobase derivatives.

TitleClickIn: a flexible protocol for quantifying mitochondrial uptake of nucleobase derivatives.
Publication TypeJournal Article
Year of Publication2017
AuthorsHoogewijs, K, James, AM, Smith, RAJ, Abendroth, F, Gait, MJ, Murphy, MP, Lightowlers, RN
JournalInterface Focus
Volume7
Issue2
Pagination20160117
Date Published2017 Apr 06
ISSN2042-8898
Abstract

There is an increasing interest in targeting molecules to the mitochondrial matrix. Many proteins are naturally imported through the translocase complexes found in the outer and inner mitochondrial membranes. One possible means for importing molecules is therefore to use a mitochondrial pre-protein as a vector and assess what forms of molecules can be attached to the pre-protein as cargo. A major difficulty with this approach is to ensure that any chimaeric molecule does indeed access the mitochondrial matrix and does not merely associate with the mitochondrial membranes. We have recently demonstrated that click chemistry can be used both to demonstrate convincingly mitochondrial import of a peptide-peptide nucleic acid conjugate and also to quantify the mitochondrial uptake for specific synthetic conjugates. We now report an adaptation of the synthesis to facilitate simple quantification of multiple molecules and hence to calculate the efficiency of their mitochondrial import.

DOI10.1098/rsfs.2016.0117
Alternate JournalInterface Focus
Citation Key10.1098/rsfs.2016.0117
PubMed ID28382203
PubMed Central IDPMC5311907