Basal mitophagy is widespread inbut minimally affected by loss of Pink1 or parkin.

TitleBasal mitophagy is widespread inbut minimally affected by loss of Pink1 or parkin.
Publication TypeJournal Article
Year of Publication2018
AuthorsLee, JJ, Sanchez-Martinez, A, Zarate, AMartinez, Beninc√°, C, Mayor, U, Clague, MJ, Whitworth, AJ
JournalJ Cell Biol
Date Published2018 Mar 02
ISSN1540-8140
Abstract

The Parkinson's disease factors PINK1 and parkin are strongly implicated in stress-induced mitophagy in vitro, but little is known about their impact on basal mitophagy in vivo. We generated transgenicexpressing fluorescent mitophagy reporters to evaluate the impact ofmutations on basal mitophagy under physiological conditions. We find that mitophagy is readily detectable and abundant in many tissues, including Parkinson's disease-relevant dopaminergic neurons. However, we did not detect mitolysosomes in flight muscle. Surprisingly, inornull flies, we did not observe any substantial impact on basal mitophagy. Because these flies exhibit locomotor defects and dopaminergic neuron loss, our findings raise questions about current assumptions of the pathogenic mechanism associated with the PINK1/parkin pathway. Our findings provide evidence that Pink1 and parkin are not essential for bulk basal mitophagy inThey also emphasize that mechanisms underpinning basal mitophagy remain largely obscure.

DOI10.1083/jcb.201801044
Alternate JournalJ. Cell Biol.
Citation Key10.1083/jcb.201801044
PubMed ID29500189