Oxygenation of frog gastric mucosa in vitro.

TitleOxygenation of frog gastric mucosa in vitro.
Publication TypeJournal Article
Year of Publication1975
AuthorsKidder, GW, Montgomery, CW
JournalAm J Physiol
Volume229
Issue6
Pagination1510-3
Date Published1975 Dec
ISSN0002-9513
KeywordsAnimals, Anura, Atmosphere Exposure Chambers, Gastric Juice, Gastric Mucosa, Hydrogen-Ion Concentration, Hyperbaric Oxygenation, In Vitro Techniques, Membrane Potentials, Oxygen Consumption
Abstract

We have recently shown that 5% CO2/95% O2 in the serosal bathing solution, with 100% O2 in the mucosal solution, results in CO2-diffusion limitation of acid secretion in bullfrog gastric mucosa. Changing to 10% CO2/90% 02 on both surfaces doubles the acid secretory rate. We calculate that, were the rate of oxygen consumption to increase significantly as a result of secretory stimulation, the tissue would now be oxygen limited. This prediction is tested by raising the P02 by increasing the total pressure in a hyperbaric chamber. Since no change in acid secretory rate or potential difference was observed upon changing from PO2 = 0.9 to PO2 = 1.9 atm, we conclude that the tissue is not O2 limited at normal pressure. Decreasing PO2 below 0.9 atm, by contrast, decreases the acid secretory rate and raises both PD and resistance. We infer that the rate of oxygen consumption did not rise significantly when acid secretion was increased by supplying sufficient CO2.

DOI10.1152/ajplegacy.1975.229.6.1510
Alternate JournalAm. J. Physiol.
Citation Key10.1152/ajplegacy.1975.229.6.1510
PubMed ID2018