Somatic variants in autosomal dominant genes are a rare cause of sporadic Alzheimer's disease.

TitleSomatic variants in autosomal dominant genes are a rare cause of sporadic Alzheimer's disease.
Publication TypeJournal Article
Year of Publication2018
AuthorsNicolas, G, Acuna-Hidalgo, R, Keogh, MJ, Quenez, O, Steehouwer, M, Lelieveld, S, Rousseau, S, Richard, A-C, Oud, MS, Marguet, F, Laquerrière, A, Morris, CM, Attems, J, Smith, C, Ansorge, O, Sarraj, SAl, Frebourg, T, Campion, D, Hannequin, D, Wallon, D, Gilissen, C, Chinnery, PF, Veltman, JA, Hoischen, A
JournalAlzheimers Dement
Date Published2018 Aug 13
ISSN1552-5279
Abstract

INTRODUCTION: A minority of patients with sporadic early-onset Alzheimer's disease (AD) exhibit de novo germ line mutations in the autosomal dominant genes such as APP, PSEN1, or PSEN2. We hypothesized that negatively screened patients may harbor somatic variants in these genes.

METHODS: We applied an ultrasensitive approach based on single-molecule molecular inversion probes followed by deep next generation sequencing of 11 genes to 100 brain and 355 blood samples from 445 sporadic patients with AD (>80% exhibited an early onset,

RESULTS: We identified and confirmed nine somatic variants (allele fractions: 0.2%-10.8%): two APP, five SORL1, one NCSTN, and one MARK4 variant by independent amplicon-based deep sequencing.

DISCUSSION: Two of the SORL1 variant might have contributed to the disease, the two APP variants were interpreted as likely benign and the other variants remained of unknown significance. Somatic variants in the autosomal dominant AD genes may not be a common cause of sporadic AD, including early onset cases.

DOI10.1016/j.jalz.2018.06.3056
Alternate JournalAlzheimers Dement
Citation Key10.1016/j.jalz.2018.06.3056
PubMed ID30114415