Delivery of mtZFNs into Early Mouse Embryos.

TitleDelivery of mtZFNs into Early Mouse Embryos.
Publication TypeJournal Article
Year of Publication2018
AuthorsMcCann, BJ, Cox, A, Gammage, PA, Stewart, JB, Zernicka-Goetz, M, Minczuk, MA
JournalMethods Mol Biol
Volume1867
Pagination215-228
Date Published2018
ISSN1940-6029
Abstract

Mitochondrial diseases often result from mutations in the mitochondrial genome (mtDNA). In most cases, mutant mtDNA coexists with wild-type mtDNA, resulting in heteroplasmy. One potential future approach to treat heteroplasmic mtDNA diseases is the specific elimination of pathogenic mtDNA mutations, lowering the level of mutant mtDNA below pathogenic thresholds. Mitochondrially targeted zinc-finger nucleases (mtZFNs) have been demonstrated to specifically target and introduce double-strand breaks in mutant mtDNA, facilitating substantial shifts in heteroplasmy. One application of mtZFN technology, in the context of heteroplasmic mtDNA disease, is delivery into the heteroplasmic oocyte or early embryo to eliminate mutant mtDNA, preventing transmission of mitochondrial diseases through the germline. Here we describe a protocol for efficient production of mtZFN mRNA in vitro, and delivery of these into 0.5 dpc mouse embryos to elicit shifts of mtDNA heteroplasmy.

DOI10.1007/978-1-4939-8799-3_16
Alternate JournalMethods Mol. Biol.
Citation Key10.1007/978-1-4939-8799-3_16
PubMed ID30155826