APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.

TitleAPOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.
Publication TypeJournal Article
Year of Publication2019
AuthorsSignes, A, Cerutti, R, Dickson, AS, Beninc√°, C, Hinchy, EC, Ghezzi, D, Carrozzo, R, Bertini, E, Murphy, MP, Nathan, JA, Viscomi, C, Fernandez-Vizarra, E, Zeviani, M
JournalEMBO Mol Med
Volume11
Issue1
Date Published2019 Jan
ISSN1757-4684
Abstract

Loss-of-function mutations in , a gene exclusively found in higher eukaryotes, cause a characteristic type of cavitating leukoencephalopathy associated with mitochondrial cytochrome oxidase (COX) deficiency. Although the genetic association of APOPT1 pathogenic variants with isolated COX defects is now clear, the biochemical link between APOPT1 function and COX has remained elusive. We investigated the molecular role of APOPT1 using different approaches. First, we generated an knockout mouse model which shows impaired motor skills, e.g., decreased motor coordination and endurance, associated with reduced COX activity and levels in multiple tissues. In addition, by achieving stable expression of wild-type APOPT1 in control and patient-derived cultured cells we ruled out a role of this protein in apoptosis and established instead that this protein is necessary for proper COX assembly and function. On the other hand, APOPT1 steady-state levels were shown to be controlled by the ubiquitination-proteasome system (UPS). Conversely, in conditions of increased oxidative stress, APOPT1 is stabilized, increasing its mature intramitochondrial form and thereby protecting COX from oxidatively induced degradation.

DOI10.15252/emmm.201809582
Alternate JournalEMBO Mol Med
Citation Key10.15252/emmm.201809582
PubMed ID30552096
PubMed Central IDPMC6328941