Inhibition of the deubiquitinase USP8 corrects a Drosophila PINK1 model of mitochondria dysfunction.

TitleInhibition of the deubiquitinase USP8 corrects a Drosophila PINK1 model of mitochondria dysfunction.
Publication TypeJournal Article
Year of Publication2019
Authorsvon Stockum, S, Sanchez-Martinez, A, Corrà, S, Chakraborty, J, Marchesan, E, Locatello, L, Da Re, C, Cusumano, P, Caicci, F, Ferrari, V, Costa, R, Bubacco, L, Rasotto, MBerica, Szabó, I, Whitworth, AJ, Scorrano, L, Ziviani, E
JournalLife Sci Alliance
Volume2
Issue2
Date Published2019 Apr
ISSN2575-1077
Abstract

Aberrant mitochondrial dynamics disrupts mitochondrial function and contributes to disease conditions. A targeted RNA interference screen for deubiquitinating enzymes (DUBs) affecting protein levels of multifunctional mitochondrial fusion protein Mitofusin (MFN) identified USP8 prominently influencing MFN levels. Genetic and pharmacological inhibition of USP8 normalized the elevated MFN protein levels observed in PINK1 and Parkin-deficient models. This correlated with improved mitochondrial function, locomotor performance and life span, and prevented dopaminergic neurons loss in PINK1 KO flies. We identified a novel target antagonizing pathologically elevated MFN levels, mitochondrial dysfunction, and dopaminergic neuron loss of a model of mitochondrial dysfunction.

DOI10.26508/lsa.201900392
Alternate JournalLife Sci Alliance
Citation Key10.26508/lsa.201900392
PubMed ID30988163
PubMed Central IDPMC6467245
Grant List / / Wellcome Trust / United Kingdom