Detection of changes in mitochondrial hydrogen sulfide in the fish model (Poeciliidae).

TitleDetection of changes in mitochondrial hydrogen sulfide in the fish model (Poeciliidae).
Publication TypeJournal Article
Year of Publication2019
AuthorsLau, GY, Barts, N, Hartley, RC, Tobler, M, Richards, JG, Murphy, MP, Arndt, S
JournalBiol Open
Volume8
Issue5
Date Published2019 May 09
ISSN2046-6390
Abstract

In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect changes in mitochondrial hydrogen sulfide (HS) in (family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with HS to form the product MitoN. The MitoN/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial HS produced over time. We describe the use of MitoA in the fish species to illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues followed by tandem mass spectrometry. In this proof-of-concept study we exposed HS tolerant to 59 µM free HS for 5 h, which resulted in increased MitoN/MitoA in brain and gills, but not in liver or muscle, demonstrating increased mitochondrial HS levels in select tissues following whole-animal HS exposure. This is the first time that accumulation of HS has been observed during whole-animal exposure to free HS using MitoA. This article has an associated First Person interview with the first author of the paper.

DOI10.1242/bio.041467
Alternate JournalBiol Open
Citation Key10.1242/bio.041467
PubMed ID31072908
PubMed Central IDPMC6550084
Grant List / / Wellcome Trust / United Kingdom