Species and tissue specific differences in ROS metabolism to hypoxia- and hyperoxia-recovery exposure in marine sculpins.

TitleSpecies and tissue specific differences in ROS metabolism to hypoxia- and hyperoxia-recovery exposure in marine sculpins.
Publication TypeJournal Article
Year of Publication2019
AuthorsLau, GY, Arndt, S, Murphy, MP, Richards, JG
JournalJ Exp Biol
Date Published2019 Oct 18
ISSN1477-9145
Abstract

Animals that inhabit environments that fluctuate in oxygen must not only contend with disruptions to aerobic metabolism, but also the potential effects of reactive oxygen species (ROS) generation. The goal of this study was to compare aspects of ROS metabolism in response to O variability (6 hr hypoxia or hyperoxia, with subsequent normoxic recovery) in two species of intertidal sculpin fishes (Cottidae, Actinopterygii) that can experience O fluctuations in their natural environment and differ in whole animal hypoxia tolerance. To assess ROS metabolism, we measured the ratio of glutathione and glutathione disulfide as an indicator of tissue redox environment, MitoP/MitoB ratio to assess mitochondrial ROS generation, thiobarbituric acid reactive substances (TBARS) for lipid peroxidation, and total oxidative scavenging capacity (TOSC) in the liver, brain, and gill. In the brain, the more hypoxia tolerant showed large increases in TBARS levels following hypoxia and hyperoxia exposure that were generally not associated with large changes in mitochondrial HO In contrast, the less-tolerant showed no significant changes in TBARS or mitochondrial HO in the brain. More moderate responses were observed in the liver and gill of exposed to hypoxia and hyperoxia with normoxic recovery, whereas showed more responses to O variability in these tissues. Our results show that the relationship between hypoxia tolerance and ROS metabolism is species and tissue specific.

DOI10.1242/jeb.206896
Alternate JournalJ. Exp. Biol.
Citation Key10.1242/jeb.206896
PubMed ID31628206