|Title||Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Hirst, J, Kunji, ERS, Walker, JE|
|Date Published||2019 11 08|
|Keywords||Lipids, Mass Spectrometry, Membrane Proteins|
Chorev (Reports, 16 November 2018, p. 829) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive and lack experimental validation. The proteins are not in their "native" condition: They have been stripped of tightly bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.