Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".

TitleComment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".
Publication TypeJournal Article
Year of Publication2019
AuthorsHirst, J, Kunji, ERS, Walker, JE
JournalScience
Volume366
Issue6466
Date Published2019 11 08
ISSN1095-9203
KeywordsLipids, Mass Spectrometry, Membrane Proteins
Abstract

Chorev (Reports, 16 November 2018, p. 829) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive and lack experimental validation. The proteins are not in their "native" condition: They have been stripped of tightly bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.

DOI10.1126/science.aaw9830
Alternate JournalScience
Citation Key10.1126/science.aaw9830
PubMed ID31699909