A sensitive mass spectrometric assay for mitochondrial CoQ pool redox state in vivo.

TitleA sensitive mass spectrometric assay for mitochondrial CoQ pool redox state in vivo.
Publication TypeJournal Article
Year of Publication2019
AuthorsBurger, N, Logan, A, Prime, TA, Mottahedin, A, Caldwell, ST, Krieg, T, Hartley, RC, James, AM, Murphy, MP
JournalFree Radic Biol Med
Date Published2019 Dec 05
ISSN1873-4596
Abstract

Coenzyme Q (CoQ) is an essential cofactor, primarily found in the mitochondrial inner membrane where it functions as an electron carrier in the respiratory chain, and a lipophilic antioxidant. The redox state of the CoQ pool is the ratio of its oxidised (ubiquinone) and reduced (ubiquinol) forms, and is a key indicator of mitochondrial bioenergetic and antioxidant status. However, the role of CoQ redox state in vivo is poorly understood, because determining its value is technically challenging due to redox changes during isolation, extraction and analysis. To address these problems, we have developed a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay that enables us to extract and analyse both the CoQ redox state and the magnitude of the CoQ pool with negligible changes to redox state from small amounts of tissue. This will enable the physiological and pathophysiological roles of the CoQ redox state to be investigated in vivo.

DOI10.1016/j.freeradbiomed.2019.11.028
Alternate JournalFree Radic. Biol. Med.
Citation Key10.1016/j.freeradbiomed.2019.11.028
PubMed ID31811922