Immunological Synapse Formation Induces Mitochondrial Clustering and Mitophagy in Dendritic Cells

TitleImmunological Synapse Formation Induces Mitochondrial Clustering and Mitophagy in Dendritic Cells
Publication TypeJournal Article
Year of Publication2019
AuthorsGómez-Cabañas, L, López-Cotarelo, P, Criado-García, O, Murphy, MP, Boya, P, Rodríguez-Fernández, JLuis
JournalThe Journal of Immunology
Pagination1715 - 1723
Date PublishedMar-03-2020

The immunological synapse (IS) is a superstructure formed during T cell activation at the zone of contact between T cells and dendritic cells (DCs). The IS includes specific molecular components in the T cell and DCs sides that may result in different functionality. Most of the studies on the IS have focused on the T cell side of this structure and, in contrast, the information available on the IS of DCs is sparse. Autophagy is a cellular process involved in the clearance of damaged proteins and organelles via lysosomal degradation. Mitophagy is the selective autophagy of damaged mitochondria. In this study, it is shown that IS formation induces clustering of mitochondria in the IS of DCs and partial depolarization of these organelles. At the IS of the DCs also accumulate autophagy and mitophagy markers, even when the kinase complex mTORC1, an inhibitor of the autophagy, is active. Together the results presented indicate that IS formation induces local clustering of mitochondria and mitophagy, which could be a homeostatic mechanism to control the quality of mitochondria in this region. The data underline the complexity of the regulatory mechanisms operating in the IS of DCs.

Short TitleJ.I.
Citation Key10.4049/jimmunol.1800575