Antioxidant properties of MitoTEMPOL and its hydroxylamine.

TitleAntioxidant properties of MitoTEMPOL and its hydroxylamine.
Publication TypeJournal Article
Year of Publication2009
AuthorsTrnka, J, Blaikie, FH, Logan, A, Smith, RAJ, Murphy, MP
JournalFree Radic Res
Volume43
Issue1
Pagination4-12
Date Published2009 Jan
ISSN1029-2470
KeywordsAnimals, Antioxidants, Cyclic N-Oxides, DNA, Mitochondrial, Hydroxylamine, Lipid Peroxidation, Mice, Mitochondria, Liver, Oxidation-Reduction, Polymerase Chain Reaction, Rats, Spin Labels, Superoxide Dismutase
Abstract

Piperidine nitroxides such as TEMPOL have been widely used as antioxidants in vitro and in vivo. MitoTEMPOL is a mitochondria-targeted derivative of TEMPOL designed to protect mitochondria from the oxidative damage that they accumulate, but once there is rapidly reduced to its hydroxylamine, MitoTEMPOL-H. As little is known about the antioxidant efficacy of hydroxylamines, this study has assessed the antioxidant activity of both MitoTEMPOL and MitoTEMPOL-H. The hydroxylamine was more effective at preventing lipid-peroxidation than MitoTEMPOL and decreased oxidative damage to mitochondrial DNA caused by menadione. In contrast to MitoTEMPOL, MitoTEMPOL-H has no superoxide dismutase activity and its antioxidant actions are likely to be mediated by hydrogen atom donation. Therefore, even though MitoTEMPOL is rapidly reduced to MitoTEMPOL-H in cells, it remains an effective antioxidant. Furthermore, as TEMPOL is also reduced to a hydroxylamine in vivo, many of its antioxidant effects may also be mediated by its hydroxylamine.

DOI10.1080/10715760802582183
Alternate JournalFree Radic. Res.
Citation Key10.1080/10715760802582183
PubMed ID19058062
PubMed Central IDPMC2645131
Grant ListMC_U105663142 / / Medical Research Council / United Kingdom