Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.

TitleDynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.
Publication TypeJournal Article
Year of Publication2008
AuthorsAzzu, V, Affourtit, C, Breen, EP, Parker, N, Brand, MD
JournalBiochim Biophys Acta
Volume1777
Issue10
Pagination1378-83
Date Published2008 Oct
ISSN0006-3002
KeywordsAnimals, Cell Line, Tumor, Glucose, Insulinoma, Ion Channels, Mitochondria, Mitochondrial Proteins, Rats, Uncoupling Protein 2
Abstract

Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.

DOI10.1016/j.bbabio.2008.07.001
Alternate JournalBiochim. Biophys. Acta
Citation Key10.1016/j.bbabio.2008.07.001
PubMed ID18692019
PubMed Central IDPMC2568827
Grant ListMC_U105663137 / / Medical Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom