Dissociation of superoxide production by mitochondrial complex I from NAD(P)H redox state.

TitleDissociation of superoxide production by mitochondrial complex I from NAD(P)H redox state.
Publication TypeJournal Article
Year of Publication2008
AuthorsLambert, AJ, Buckingham, JA, Brand, MD
JournalFEBS Lett
Volume582
Issue12
Pagination1711-4
Date Published2008 May 28
ISSN0014-5793
KeywordsAnimals, Electron Transport Complex I, Female, Flavins, Mitochondria, Muscle, NADP, Oxidation-Reduction, Rats, Rats, Wistar, Superoxides
Abstract

The relationship between the rate of superoxide production by complex I and NAD(P)H redox state was investigated in rat skeletal muscle mitochondria. A high rate of superoxide production was observed during succinate oxidation; the rate during pyruvate oxidation was over fourfold lower. However, the NAD(P)H pool was significantly less reduced during succinate oxidation than during pyruvate oxidation. We conclude that there is no unique relationship between superoxide production by complex I and the reduction state of the NAD(P)H pool. Our data suggest that less than 10% of the superoxide originates from the flavin site during reverse electron transport from succinate.

DOI10.1016/j.febslet.2008.04.030
Alternate JournalFEBS Lett.
Citation Key10.1016/j.febslet.2008.04.030
PubMed ID18442479
Grant List066750 / / Wellcome Trust / United Kingdom
MC_U105663137 / / Medical Research Council / United Kingdom
066750/B/01/Z / / Wellcome Trust / United Kingdom